Improving Medical Devices With Nanobubbles

Scientists have discovered an ingenious way to improve medical device performance.

Researchers from the University of Sydney Nano Institute and School of Chemistry (Australia) have found that tiny gas bubbles—nanobubbles just 100 billionths of a meter high—form on surfaces in unexpected situations, providing a new way to reduce drag in small-scale devices.
 
Liquid drag within microdevices can lead to internal biofouling (accumulation of unwanted biological materials) or damage biological samples such as cells, due to high pressure. Consequently, the discovery could lead engineers to develop better medical diagnostic tools such as lab-on-a-chip devices that undertake DNA analysis or are used for biomedical detection of disease pathogens, including PCR tests.
 
Led by Professor Chiara Neto, the scientific team developed nanoengineered wrinkled coatings that reduce drag by up to 38 percent compared with nominally “smooth” solid surfaces. The slippery coatings, once infused with a lubricant, are also highly resistant to biofouling. Using atomic-force microscopy—a very high-resolution scanning microscope—the team discovered that the fluids passing through micro-structured channels with these surfaces were able to slip through with lower friction due to the spontaneous formation of nanobubbles, a phenomenon never before described.

“We want to understand the fundamental mechanism by which these surfaces work and to push the boundaries of their application, especially for energy efficiency. Now that we know why these surfaces are slippery and drag-reducing, we can design them specifically to minimize the energy required to drive flow in confined geometries and reduce fouling,” Neto said.
 
The results are published in Nature Communications.
 
Potential Medical Application
Many medical diagnostic tools rely on the analysis of tiny amounts of biological and other materials in liquid form. These microfluidic devices use microchannels and microreactors that conduct reactions at a miniaturized scale. Analyzing much smaller volumes of material enables faster and more efficient diagnostics. However, microfluidic devices’ fluid flow is dramatically slowed down by the friction of the liquid within the solid walls of the channels, creating a large hydrodynamic drag. To overcome this, the devices apply high pressures to drive the flow.
 
In turn, the high pressure inside these devices is not only inefficient but can also damage delicate samples in the device, such as cells and other soft materials. Moreover, the solid walls easily become fouled by biological molecules or bacteria, leading to fast degradation through biofouling. These problems can be addressed by using surfaces in which nanoscale pores trap small amounts of a lubricant, forming a slippery liquid interface that reduces hydrodynamic drag and prevents surface fouling.
 
In effect, liquid-infused surfaces replace the solid wall with a liquid wall, allowing the flow of a second liquid with lower friction, requiring lower pressure. However, the mechanism by which these liquid-infused surfaces work has not been understood, as the reduction of friction that these surfaces offer has been reported to be 50 times larger than would be expected based on theory.
 
Nanobubbles to the Rescue?
Professor Neto and her team have described the process by which they formed liquid-infused walls on their microfluidic devices: developing nanoengineered wrinkled coatings that reduce drag by up to 38 percent compared to solid walls. The team includes Ph.D. student Chris Vega-Sánchez, whose work over the past three years focused on microfluidics; Dr Sam Peppou-Chapman, an expert in liquid-infused surfaces; and Dr Liwen Zhu, an expert in atomic force microscopy, which gives scientists the ability to see down to a billionth of a meter.
 
Conducting microfluidic measurements, the team revealed that the new slippery surfaces reduced drag relative to solid surfaces to a degree that would be expected only if the surface was infused with air rather than a viscous lubricant. Not satisfied with the successful drag reduction, the team worked to demonstrate the mechanism by which the surfaces induced slip. They did this by scanning the surfaces underwater using atomic-force microscopy, enabling them to image the spontaneous formation of nanobubbles, only 100 nanometres high on the surface. Their presence quantitatively explains the huge slip observed in microfluidic flow.
 
Part of the microscopy work was done using the facilities of the Australian Centre for Microscopy & Microanalysis at the University of Sydney.
 
The research was funded by the Australian Research Council (FT180100214), and the Costa Rican Ministry of Science and Technology.